Does the Enigmatic Clusterin Have a Chaperone-Like Activity During Sepsis in Children?

date: 19. 6. 2010, author: J. Žurek, P.Košut, M. Fedora
Name Clusterin Human ELISA
Cat. No. RD194034200R RUO
Other names Apolipoprotein J, Apo J
Product category Oncology, Others, Renal disease, Sepsis
Assay format Sandwich ELISA, Biotin-labelled antibody
Calibration range 5-160 ng/ml
Limit of detection 0.5 ng/ml
Applications Cerebrospinal fluid, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine
Sample requirements 5 µl/well
Storage/Shipping Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

J. Žurek, P.Košut, M. Fedora
Department of Anesthesia and Intensive Care, School of Medicine, Masaryk University, University Children´s Hos­pital, Czech Republic

Objectives: Clusterin is an enigma. We hypothesized that the functions of clusterin are expressed during cellular stress. The aim was to clarify the role of clusterin serum levels in children where correlated with the severity of sepsis.

Methods: Twenty-eight children (18 men and 10 women; mean age 86 ± 69 months, PELOD mean 13 ± 10) with SIRS or septic state were prospectively enrolled in the study. Blood samples were obtained from a venous catheter during the first 12 h after diagnosis, then in the course of the duration of the diagnosis of SIRS or septic state.

Results: Clusterin was highly up-regulated in survivors vs. non-survivors (median 85.04 µg/ml vs 37.36 µg/ml; p 0.05). In children with the development of Multiple Organ Dysfunction Syndrome a significant lower expression of clusterin (mean 33 µg/ml ± 22 µg/ml; median 31 µg/ml; min 1.5 µg/ml – max 80 µg/ml; p 0.0001) has been observed.

Conclusion: We have demonstrated a significant decrease in levels of clusterin in relation to the mortality and severity of sepsis.
Furthermore, understanding the genetic elements regulating clusterin induction could provide a useful tool to control expression of other genes in the setting of organ injury.

References:

1. Kalenka A, Feldmann RE Jr, Otero K, Maurer MH, Waschke KF, Fiedler F. Changes in the serum proteome of patients with sepsis and septic shock. Anesth Analg. 2006 Dec;103(­6):1522–6.

2. Steve E. Jones, Catherine Jomary. Clusterin. The International Journal of Biochemistry & Cell Biology (2002) 34;427–431

The results will be presented at the CSARIM2010 – XVIIth National Congress of the Czech Society of Anaesthesiology and Intensive Care Medicine, September 9–11, 2010, Zlin, the Czech Republic

Catalog NumberSpeciesAnalyteAssayRegulatoryFormat
RD194034200R Human Clusterin Sandwich ELISA, Biotin-labelled antibody RUO 96 wells (1 kit)
categories: Oncology, Others, Renal disease, Sepsis